Journal: The Journal of General Physiology

Article Title: A mouse model of Huntington’s disease shows altered ultrastructure of transverse tubules in skeletal muscle fibers

doi: 10.1085/jgp.202012637

Figure Lengend Snippet: Bin1 protein levels in muscles of R6/2 and control mice . (A–D) Western blots for Bin1 protein in the quadriceps femoris (A and B) and the gastrocnemius (C and D) of age-matched late-stage (10–13 wk of age) control (WT) and R6/2 mice. (A and C) Representative Western blots for Bin1 as well as β-tubulin and GAPDH for normalization. Equal amounts of protein were loaded per lane (50 µg). (B and D) Bin1 band intensities normalized to β-tubulin and GAPDH levels (relative to control) for the quadriceps femoris (B) and gastrocnemius (D). For A and B (quadriceps femoris), n = 4/group. For C and D (gastrocnemius), n = 6/group. *, Significantly different from control at P < 0.05. AU, arbitrary units.

Article Snippet: For normalization purposes, blots were probed using an anti–β-tubulin rabbit polyclonal antibody (cat. #PA5-16863; Thermo Fisher Scientific), and an anti-glyceraldehyde phosphate dehydrogenase (GAPDH) mouse monoclonal antibody (cat. #MA5-15738; Thermo Fisher Scientific).

Techniques: Western Blot

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Protective Effects of Phellinus linteus Mycelium on the Development of Osteoarthritis after Monosodium Iodoacetate Injection

doi: 10.1155/2020/7240858

Figure Lengend Snippet: NADPH oxidases: NOX4, p47 phox , and p22 phox in cartilage tissues. Values are the mean ± SEM ( n = 6). Nor: normal rats; Con; MIA-induced osteoarthritis rats; GLM200: MIA-induced osteoarthritis rats administrated with green-lipped mussel 200 mg/kg body weight; PLM50: MIA-induced osteoarthritis rats administrated with PLM 50 mg/kg body weight; PLM100: MIA-induced osteoarthritis rats administrated with PLM 100 mg/kg body weight; PLM200: MIA-induced osteoarthritis rats administrated with PLM 200 mg/kg body weight. Significance: # P < 0.05, ### P < 0.001 vs. normal rat values and ∗ P < 0.05, ∗∗ P < 0.01 vs. MIA control rat values.

Article Snippet: Moreover, rabbit polyclonal antireduced nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) was obtained from LifeSpan BioSciences (Seattle, WA, USA).

Techniques:

Journal: The Plant Cell

Article Title: Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii ^{[W] [OPEN]}

doi: 10.1105/tpc.114.126375

Figure Lengend Snippet: Adaptation of the Photosynthetic Apparatus of pgrl1 and Wild-Type C. reinhardtii Cells to a Switch from High to Low CO2. Cells were cultivated autotrophically in photobioreactors operated as turbidostats at a constant biomass concentration (≈1.5 × 106 cells mL−1) in the presence of 2% CO2-enriched air under a light intensity of 500 μmol photons m−2 s−1. Upon 48 h stabilization, cultures were shifted to air CO2 levels (0 h). Samples were taken at 0, 2, 4, 6, and 24 h after the shift in order to perform immunodetection ([A] and [B]) and functional analysis ([C] to [E]). (A) and (B) Different antibodies raised against PsaC (PSI), PsbD (PSII), PGRL1, NDA2, AOX1 (AOX), COXIIb (COXII), FeSOD, and FLVs were used to decorate immunoblots. Samples were loaded at equal total proteins amounts based on Coomassie blue staining (Control). (C) PSI/PSII ratio determined from ECS measurements. (D) Light-dependent O2 uptake rates were measured using a MIMS in the presence of [18O]-enriched O2 as in Figure 5; corresponding O2 production rates are shown as Supplemental Figure 5. (E) Growth performances measured as dilution rates used to maintain the culture at a constant biomass concentration; pgrl1 (gray line) and wild-type control (black line).

Article Snippet: Representative subunits of photosynthetic complexes were used to decorate immunoblots: anti-PsaC ( PSI ), anti-PsbD ( PSII ), anti-cytf ( cytb 6 f ), anti-AOX1 (AOX), anti-COXIIb (COXII), and anti-FeSOD, all purchased from Agrisera.

Techniques: Concentration Assay, Immunodetection, Functional Assay, Western Blot, Staining, Control

Journal: Plants

Article Title: Chemopreventive Effect of Cooked Chickpea on Colon Carcinogenesis Evolution in AOM/DSS-Induced Balb/c Mice

doi: 10.3390/plants12122317

Figure Lengend Snippet: ( A ) Percentage of iNOS expression in BALB/c mice colon at the three evaluation times. ( B ) COX-2 expression in BALB/c mice colon at three evaluation times. ( C ) Images of colon tissue from immunohistochemistry (iNOS and COX-2) at week 7. Magnification 40×. * Significant difference compared with the PC group. ▲ Significant difference compared with the NC group. One-way ANOVA ( p < 0.05), Dunnett’s test.

Article Snippet: Later, the slides were incubated overnight at 4 °C with rabbit polyclonal anti-COX-2 antibody (1:100, ABCAM ab15191) diluted in TBS (pH 7.6).

Techniques: Expressing, Immunohistochemistry

Journal: The Journal of Biological Chemistry

Article Title: Membrane Estrogen Signaling Enhances Tumorigenesis and Metastatic Potential of Breast Cancer Cells via Estrogen Receptor-?36 (ER?36) *

doi: 10.1074/jbc.M111.292946

Figure Lengend Snippet: Proposed model of rapid membrane-initiated signaling of estrogen via ERα36. E2 interacts with ERα36 at the cell membrane initiating a signaling cascade consisting of phosphatidylinositol-specific phospholipase C (PLC)-dependent cleavage of PIP2 to IP3 and DAG. IP3 signals Ca2+ release from the smooth endoplasmic reticulum, although DAG anchors PKC to the membrane. The membrane localization along with Ca2+ association with PKC leads to its activation, which can be inhibited by chelerythrine, with downstream activation of ERK1/2 mitogen-activated protein kinase (MAPK). Activation of ERK1/2 can affect cell proliferation as well as phosphorylation of transcription factors that can activate or repress gene transcription, possibly leading to expression of factors associated with metastasis and tumor aggressiveness. Taxol induces apoptosis through a mechanism involving JNK MAPK and the Bcl2-associated proteins Bax and BAD, leading to release of cytochrome c from the mitochondria. This initiates the caspase cascade involving caspases-9 and -7, eventually leading to activation of caspase-3, which causes apoptosis. E2 signaling through membrane-associated receptor prevents taxol-induced apoptosis through a mechanism that may activate Akt and cause inhibition of caspase-3.

Article Snippet: Monoclonal antibodies to cytochrome C oxidase (COX) IV were from Abcam (Cambridge, MA).

Techniques: Activation Assay, Expressing, Inhibition

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Resistin, Elastase, and Lactoferrin as Potential Plasma Biomarkers of Pediatric Inflammatory Bowel Disease Based on Comprehensive Proteomic Screens

doi: 10.1016/j.mcpro.2022.100487

Figure Lengend Snippet: Top 50 IBD plasma biomarkers from aptamer-based screening (AUC >0.8)

Article Snippet: Human Catalase ELISA kit (catalog no.: ab171572; Abcam, 1:100 dilution), Human Elastase ELISA kit (catalog no.: ab11955; Abcam, 1:25 dilution), Human Insulin-Like Growth Factor 1 (IGF-1) ELISA kit (catalog no.: ELH-IGF1; Raybiotech, 1:10 dilution), Human Lactoferrin (LTF) ELISA kit (catalog no.: ELH-LTF; Raybiotech, 1:25 dilution), Human Peroxiredoxin ELISA kit (catalog no.: ab185983; Abcam, 1:10 dilution), Human Resistin ELISA kit (catalog no.: ELH-resistin; Raybiotech, 1:10 dilution), and Human tumor necrosis factor alpha (TNF-α) ELISA kit (catalog no.: ELH-TNF-α; Raybiotech, 1:5 dilution) were used as per the manufacturer’s instructions.

Techniques:

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Resistin, Elastase, and Lactoferrin as Potential Plasma Biomarkers of Pediatric Inflammatory Bowel Disease Based on Comprehensive Proteomic Screens

doi: 10.1016/j.mcpro.2022.100487

Figure Lengend Snippet: Comparison between pediatric IBD and adult IBD

Article Snippet: Human Catalase ELISA kit (catalog no.: ab171572; Abcam, 1:100 dilution), Human Elastase ELISA kit (catalog no.: ab11955; Abcam, 1:25 dilution), Human Insulin-Like Growth Factor 1 (IGF-1) ELISA kit (catalog no.: ELH-IGF1; Raybiotech, 1:10 dilution), Human Lactoferrin (LTF) ELISA kit (catalog no.: ELH-LTF; Raybiotech, 1:25 dilution), Human Peroxiredoxin ELISA kit (catalog no.: ab185983; Abcam, 1:10 dilution), Human Resistin ELISA kit (catalog no.: ELH-resistin; Raybiotech, 1:10 dilution), and Human tumor necrosis factor alpha (TNF-α) ELISA kit (catalog no.: ELH-TNF-α; Raybiotech, 1:5 dilution) were used as per the manufacturer’s instructions.

Techniques:

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Resistin, Elastase, and Lactoferrin as Potential Plasma Biomarkers of Pediatric Inflammatory Bowel Disease Based on Comprehensive Proteomic Screens

doi: 10.1016/j.mcpro.2022.100487

Figure Lengend Snippet: Significant biomarkers of pediatric IBD compared with adult IBD parameters

Article Snippet: Human Catalase ELISA kit (catalog no.: ab171572; Abcam, 1:100 dilution), Human Elastase ELISA kit (catalog no.: ab11955; Abcam, 1:25 dilution), Human Insulin-Like Growth Factor 1 (IGF-1) ELISA kit (catalog no.: ELH-IGF1; Raybiotech, 1:10 dilution), Human Lactoferrin (LTF) ELISA kit (catalog no.: ELH-LTF; Raybiotech, 1:25 dilution), Human Peroxiredoxin ELISA kit (catalog no.: ab185983; Abcam, 1:10 dilution), Human Resistin ELISA kit (catalog no.: ELH-resistin; Raybiotech, 1:10 dilution), and Human tumor necrosis factor alpha (TNF-α) ELISA kit (catalog no.: ELH-TNF-α; Raybiotech, 1:5 dilution) were used as per the manufacturer’s instructions.

Techniques: